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mouse fetuin a ahsg duoset elisa  (R&D Systems)


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    R&D Systems mouse fetuin a ahsg duoset elisa
    Mouse Fetuin A Ahsg Duoset Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+fetuin+a+ahsg+duoset+elisa/pmc12073913-213-5-9?v=R%26D+Systems
    Average 93 stars, based on 6 article reviews
    mouse fetuin a ahsg duoset elisa - by Bioz Stars, 2026-08
    93/100 stars

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    Regulatory role of fetuin-A in adaptive immunity following pulmonary Chlamydia infection. Age-matched wild-type (WT) C57BL/6 and fetuin-A-deficient AHSG mice were challenged intranasally with 500 IFU Cm. (a) Splenocyte IFN- γ secretion ( n = 6 per group) from day 12 after Cm challenged animals stimulated with medium alone, UV-inactivated Cm, or anti-CD3 antibody. IFN- γ was determined 72 hours poststimulation. (b) Antichlamydial protease-like activity factor (CPAF) elicited titers. Serum ( n = 6 per group) was collected at day 12 after Cm challenge. Preparation of splenocytes, IFN- γ quantitation by sandwich <t>ELISA,</t> collection of serum, and determination of CPAF titer were carried out as described under “Materials and Methods.” Respective bars represent the mean ± SD. Differences between indicated groups were analysed by one-way ANOVA with Tukey's multiple comparison test. ∗∗∗∗ P < 0.0001. ns, differences not statistically significant as determined by the two-tailed Student' t -test.
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    Regulatory role of fetuin-A in adaptive immunity following pulmonary Chlamydia infection. Age-matched wild-type (WT) C57BL/6 and fetuin-A-deficient AHSG mice were challenged intranasally with 500 IFU Cm. (a) Splenocyte IFN- γ secretion ( n = 6 per group) from day 12 after Cm challenged animals stimulated with medium alone, UV-inactivated Cm, or anti-CD3 antibody. IFN- γ was determined 72 hours poststimulation. (b) Antichlamydial protease-like activity factor (CPAF) elicited titers. Serum ( n = 6 per group) was collected at day 12 after Cm challenge. Preparation of splenocytes, IFN- γ quantitation by sandwich <t>ELISA,</t> collection of serum, and determination of CPAF titer were carried out as described under “Materials and Methods.” Respective bars represent the mean ± SD. Differences between indicated groups were analysed by one-way ANOVA with Tukey's multiple comparison test. ∗∗∗∗ P < 0.0001. ns, differences not statistically significant as determined by the two-tailed Student' t -test.
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    Regulatory role of fetuin-A in adaptive immunity following pulmonary Chlamydia infection. Age-matched wild-type (WT) C57BL/6 and fetuin-A-deficient AHSG mice were challenged intranasally with 500 IFU Cm. (a) Splenocyte IFN- γ secretion ( n = 6 per group) from day 12 after Cm challenged animals stimulated with medium alone, UV-inactivated Cm, or anti-CD3 antibody. IFN- γ was determined 72 hours poststimulation. (b) Antichlamydial protease-like activity factor (CPAF) elicited titers. Serum ( n = 6 per group) was collected at day 12 after Cm challenge. Preparation of splenocytes, IFN- γ quantitation by sandwich ELISA, collection of serum, and determination of CPAF titer were carried out as described under “Materials and Methods.” Respective bars represent the mean ± SD. Differences between indicated groups were analysed by one-way ANOVA with Tukey's multiple comparison test. ∗∗∗∗ P < 0.0001. ns, differences not statistically significant as determined by the two-tailed Student' t -test.

    Journal: International Journal of Microbiology

    Article Title: Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection

    doi: 10.1155/2022/6082140

    Figure Lengend Snippet: Regulatory role of fetuin-A in adaptive immunity following pulmonary Chlamydia infection. Age-matched wild-type (WT) C57BL/6 and fetuin-A-deficient AHSG mice were challenged intranasally with 500 IFU Cm. (a) Splenocyte IFN- γ secretion ( n = 6 per group) from day 12 after Cm challenged animals stimulated with medium alone, UV-inactivated Cm, or anti-CD3 antibody. IFN- γ was determined 72 hours poststimulation. (b) Antichlamydial protease-like activity factor (CPAF) elicited titers. Serum ( n = 6 per group) was collected at day 12 after Cm challenge. Preparation of splenocytes, IFN- γ quantitation by sandwich ELISA, collection of serum, and determination of CPAF titer were carried out as described under “Materials and Methods.” Respective bars represent the mean ± SD. Differences between indicated groups were analysed by one-way ANOVA with Tukey's multiple comparison test. ∗∗∗∗ P < 0.0001. ns, differences not statistically significant as determined by the two-tailed Student' t -test.

    Article Snippet: Lung lysate fetuin-A content was determined using the mouse fetuin-A DuoSet ELISA kit (R&D Systems, Minneapolis, MN) following manufacturer's instructions.

    Techniques: Infection, Activity Assay, Quantitation Assay, Sandwich ELISA, Comparison, Two Tailed Test